Pharmacy Scholarship: Joe Deweese

Collier S, Uroza SF, McCormac M, Deweese JE, Akers WS. Connecting antibody structure and function to pharmacy practice through a point-of-care testing lab. Am J Pharm Educ. 2020;84(6):ajpe8220. Research and Education Posters Presented at the 121st Virtual Annual Meeting of the American Association of Colleges of Pharmacy, July 13-31, 2020. doi:10.5688/ajpe8220

Murphy MB, Kumar P, Bradley AM, Barton CE, Deweese JE, Mercer SL. Synthesis and evaluation of etoposide and podophyllotoxin analogs against topoisomerase IIα and HCT-116 cells. Bioorg Med Chem. 2020;28(22):115773. doi:10.1016/j.bmc.2020.115773

Keck JM, Conner JD, Wilson JT, Jiang X, Lisic EC, Deweese JE. Clarifying the mechanism of copper(II) α-(N)-heterocyclic thiosemicarbazone complexes on DNA topoisomerase IIα and IIβ. Chem Res Toxicol. 2019;32(10):2135-2143. doi:10.1021/acs.chemrestox.9b00311

Keck JM, Connor JD, Jiang X, Lisic EC, Deweese JE. Examination of the effect of copper (II) α-(N)-heterocyclic thiosemicarbazone complexes on DNA topoisomerase IIbeta. The FASEB Journal. 2019;33(1_supplement):470.475-470.475. doi: 10.1096/fasebj.2019.33.1_supplement.470.5

Fief CA, Hoang KG, Phipps SD, Wallace JL, Deweese JE. Examining the impact of antimicrobial fluoroquinolones on human DNA topoisomerase IIα and IIβ. ACS Omega. 2019;4(2):4049-4055. doi: 10.1021/acsomega.8b03428

Keck JM, Conner JD, Jiang X, Lisic EC, Deweese JE. Exploring the effects of copper (II) α-(N)-heterocyclic thiosemicarbazone complexes on human DNA topoisomerase
IIß. Am J Pharm Educ. 2019;83(5):1084. doi: 10.5688/ajpe7654

Deweese JE, Hoang KG, Rhodes J, Cordova S, Nelson CW. Progress toward mapping the domain functions of the C-terminus of human type II topoisomerases. Paper presented at: Christian Scholars’ Conference; June 5-7, 2019; Lubbock, TX.

Morris WH, Ngo L, Wilson JT, Medawala, W, Brown AR, Conner JD, Fabunmi F, Cashman DJ, Lisic EC, Yu T, Deweese JE, Jiang X. Structural and metal ion effects on human topoisomerase IIalpha inhibition by alpha-(N)-heterocyclic thiosemicarbazones. Chem Res Toxicol. 2019;32(1):90-99. doi: 10.1021/acs.chemrestox.8b00204

Deweese JE, Hoang KG, Menzie RA, Fief CA, Ayes CA, Keck JM, Wilson JT, Cordova S, Nelson CW. The variable C-terminal domain of human type II topoisomerases as a functionally relevant therapeutic target. The FASEB Journal. 2019;33(1_supplement):793.794-793.794. doi: 10.1096/fasebj.2019.33.1_supplement.793.4

Deweese JE. Applied Christian Values in pharmacy—a longitudinal course in values, ethics, and compassion. Paper presented at: Christian Scholars’ Conference; June 6-8, 2018; Nashville, TN.

Wilson JT, Fief CA, Jackson KD, Mercer SL, Deweese JE. HU-331 and oxidized cannabidiol act as inhibitors of human topoisomerase IIalpha and beta. Chem Res Toxicol. 2018;31(2):137-144. doi: 10.1021/acs.chemrestox.7b00302

Deweese JE, Fief CA, Menzie RA. Mapping the interactions and post- translational modifications of topoisomerase II. Paper presented at: Christian Scholars’ Conference; June 6-8, 2018; Nashville, TN.

Murphy MB, Kumar P, Bradley AM, Barton CE, Deweese JE, Mercer SL. Synthesis and evaluation of etoposide and podophyllotoxin analogs against topoisomerase IIα activity and in cancer cells. Am J Pharm Educ. 2018;82(5):586. doi: 10.5688/ajpe7158

Deweese JE, Nichols E, Nichols R, Casey KL. Application of the layered model as a means for describing complex cellular processes. Paper presented at: Christian Scholars’ Conference; June 7-9, 2017; Nashville, TN.

Deweese JE. Compassionate care in the classroom: Helping future pharmacists understand their patients. Poster presented at: Stanford Medicine X; April 2017; Palo Alto, CA.

Wilson JT, Jaskcon KD, Mercer SL, Deweese JE. Exploring the metabolism and topoisomerase II inhibitory activity of cannabidiol. Am J Pharm Educ. 2016;80(5):13-14. doi: 10.5688/ajpe805S2

Murphy MB, Mercer SL, Deweese JE. Inhibitors and poisons of mammalian type II topoisomerases. In: Advances in molecular toxicology. Vol 11. Elsevier; 2017:203-240.

Pace AC, Greene J, Deweese JE, et al. Measuring pharmacy student attitudes toward prayer: The Student Prayer Attitude Scale (SPAS). Christian Higher Education. 2017;16(4):200-210. doi: 10.1080/15363759.2016.1250683

Nichols E, Nichols R, Deweese JE, Casey KL. Overview of the layered model as a means for describing complex cellular processes. Paper presented at: Christian Scholars’ Conference; June 7-9, 2017; Nashville, TN.

Wilson JT, Fief CA, Jaskcon KD, Mercer SL, Deweese JE. Oxidized products of cannabidiol inhibit topoisomerase IIa. Am J Pharm Educ. 2017;81(5). doi: 10.5688/ajpe815S5

Deweese JE. A scientific analysis of Adam and the genome. Sufficient Evidence: A Journal of Christian Apologetics. 2017;7(2):131-157.

Murphy M, Deweese JE, Mercer SL. Targeting topoisomerase II: Synthesis and evaluation of halogenated podophyllotoxin and etoposide analogs. Am J Pharm Educ. 2017;81(5). doi: 10.5688/ajpe815S5

Deweese JE. Working session to create a secure biochemistry question bank: DNA metabolism session. Paper presented at: Association of Biochemistry Educators Annual Meeting; May 7-11, 2017; Clearwater Beach, FL.

Conner JD, Medawala W, Stephens MT, Morris WH, Deweese JE, Kent PL, Rice JJ, Jiang X, Lisic EC. Cu (II) benzoylpyridine thiosemicarbazone complexes: inhibition of human topoisomerase IIα and activity against breast cancer cells. Open Journal of Inorganic Chemistry. 2016;6(02):146. doi: 10.4236/ojic.2016.62010

Deweese JE. DNA topoisomerases: The 'relaxers' and 'unknotters' of the genome. Journal of Creation. 2016;30(2):92-101.

Wilson JT, Jiang X, McGill BC, Lisic EC, Deweese JE. Examination of the impact of copper(II) alpha-(N)-heterocyclic thiosemicarbazone complexes on DNA topoisomerase IIα. Chem Res Toxicol. 2016;29(4):649-658. doi: 10.1021/acs.chemrestox.5b00471

Wilson JT, Jaskcon KD, Mercer SL, Deweese JE. Exploring the metabolism and topoisomerase II inhibitory activity of cannabidiol. Am J Pharm Educ. 2016;80(5):13-14. doi: 10.5688/ajpe805S2

Wilson JT, Fief CA, Jaskcon KD, Mercer SL, Deweese JE. Oxidized products of cannabidiol inhibit topoisomerase IIα. Poster presented at: American Association of Pharmaceutical Scientists Conference; November 2016; Denver, CO.

Gibson EG, King MM, Mercer SL, Deweese JE. Two-mechanism model for the interaction of etoposide quinone with topoisomerase IIalpha. Chem Res Toxicol. 2016;29(9):1541-1548. doi: 10.1021/acs.chemrestox.6b00209

Deweese JE. The unknotters of the genome: Exploring the design of DNA topoisomerases. Paper presented at: Biological Information, Genetics, and God (BIGG) Research Retreat; 2016.

Deweese JE. DNA topoisomerases: Molecular scissors and unknotters of the genome. Poster presented at: Creation Research Society Conference; 2015; Dallas, TX.

Gibson EG, King MM, Sansom MJ, McDonald WH, Mercer SL, Deweese JE. Examination of the Topoisomerase II Covalent Poisoning Mechanism of Etoposide Quinone. Am J Pharm Educ. 2015;79(5):129. Meeting Abstracts Presented at the 116th Annual Meeting of the American Association of Colleges of Pharmacy, July 11-15, 2015. doi:10.5688/ajpe795S4

Wilson JT, McGill BC, Lisic EC, Deweese JE. Impact of thiosemicarbazones on the activity of topoisomerase IIα. Am J Pharm Educ. 2015;79(5). doi: 10.5688/ajpe795S4

Deweese JE. Q&A: How do creationists explain these facts? Reason & Revelation. 2015;35(6).

Deweese JE. What is horizontal gene transfer and does it support evolution? Reason & Revelation. 2015;35(9)

Lindsey RH, Jr., Pendleton M, Ashley RE, Mercer SL, Deweese JE, Osheroff N. Catalytic core of human topoisomerase IIα: Insights into enzyme-DNA interactions and drug mechanism. Biochemistry. 2014;53(41):6595-6602. doi: 10.1021/bi5010816

Regal K, Mercer SL, Deweese JE. Characterization of HU-331 as a cannabinoid quinone inhibitor of topoisomerase II. The FASEB Journal. 2014;28(1_supplement):549.546. doi: 10.1096/fasebj.28.1_supplement.549.6

Smith NA, Byl JA, Mercer SL, Deweese JE, Osheroff N. Etoposide quinone is a covalent poison of human topoisomerase IIbeta. Biochemistry. 2014;53(19):3229-3236. doi: 10.1021/bi500421q

Sansom MJ, Deweese JE. Fluorescence-based oligonucleotide DNA cleavage system for topoisomerase II. Poster presented at: Southeastern Regional Meeting of the American Chemical Society; October 2014; Nashville, TN.

Gibson EG, Straughn LY, Deweese JE. Generation and characterization of topoisomerase IIα mutants. The FASEB Journal. 2014;28(1_supplement):549.545. doi: 10.1096/fasebj.28.1_supplement.549.5

Regal KM, Mercer SL, Deweese JE. HU-331 is a catalytic inhibitor of topoisomerase IIalpha. Chem Res Toxicol. 2014;27(12):2044-2051.

Regal KM, Mercer SL, Deweese JE. HU-331 is a redox-dependent inhibitor of topoisomerase II. Am J Pharm Educ. 2014;78(5):4. doi: 10.5688/ajpe785111

King MM, Mercer SL, Deweese JE. Impact of etoposide quinone on DNA binding and ligation by topoisomerase IIα. Poster presented at: Southeastern Regional Meeting of the American Chemical Society; October 2014; Nashville, TN.

Wilson JT, McGill BC, Lisic EC, Deweese JE. Impact of thiosemicarbazones on the activity of topoisomerase IIα. Poster presented at: Southeastern Regional Meeting of the American Chemical Society; October 2014; Nashville, TN.

Gibson EG, Mercer SL, Deweese JE. Using etoposide resistant mutants to characterize etoposide quinone. Am J Pharm Educ. 2014;78(5). doi: 10.5688/ajpe785111

Deweese JE. Why are we still studying the genome: A review of recent findings. Paper presented at: Christian Scholars’ Conference; June 5-6, 2014; Nashville, TN.

Regal KM, Mercer SL, Deweese JE. Characterization of HU-331 as a cannabinoid quinone inhibitor of topoisomerase II. Am J Pharm Educ. 2013;77(5). doi: 10.5688/ajpe775109

Gibson EG, Deweese JE. Covalent poisons of topoisomerase II. Curr Top Pharmacol. 2013;17:1-12.

Deweese JE, Jacob DA, Gibson EG, Mercer SL. Etoposide catechol is an oxidizable topoisomerase II poison. The FASEB Journal. 2013;27(1_supplement):lb75. doi: 10.1096/fasebj.27.1_supplement.lb75

Jacob DA, Gibson EG, Mercer SL, Deweese JE. Etoposide catechol is an oxidizable topoisomerase II poison. Chem Res Toxicol. 2013;26(8):1156-1158. doi: 10.1021/tx400205n

Gibson EG, Straughn LY, Deweese JE. Generation of topoisomerase IIα mutants with altered etoposide sensitivity. Am J Pharm Educ. 2013;77(5):5. doi: 10.5688/ajpe775109

Akers WS, Deweese JE, Fowler MW, Mercer SL. Back to the basics: Integrating curricular content in the pharmaceutical sciences through laboratory based exercises. Poster presented at: American Association of Colleges of Pharmacy Annual Meeting; July 14-18, 2012; Kissimmee, FL.

Lee S, Jung SR, Heo K, Byl JA, Deweese JE, Osheroff N, Hohng S. DNA cleavage and opening reactions of human topoisomerase IIalpha are regulated via Mg2+-mediated dynamic bending of gate-DNA. Proc Natl Acad Sci U S A. 2012;109(8):2925-2930. doi: 10.1073/pnas.1115704109

Gibson EG, Jacob DA, Mercer SL, Deweese JE. Etoposide metabolites as topoisomerase II poisons. Am J Pharm Educ. 2012;76(5):3. doi: 10.5688/ajpe76599

Deweese JE. Reconciling the evidence: Design and decay? Paper presented at: Christian Scholars’ Conference; June 7-9, 2012; Nashville, TN.

Deweese JE. A team-based "enzyme workshop" for teaching enzyme function and pharmacologic targeting to first-year pharmacy students. Am J Pharm Educ. 2012;76(5):1. doi: 10.5688/ajpe76599

Fowler MW, Deweese JE, Dilks HH, Mercer SL, Phipps SD, McCormack J, Akers WS. Development of an integrated biomedical and pharmaceutical sciences teaching laboratory. Am J Pharm Educ. 2011;75(5). doi: 10.5688/ajpe755105

Jacob DA, Mercer SL, Osheroff N, Deweese JE. Etoposide quinone is a redox-dependent topoisomerase II poison. Biochemistry. 2011;50(25):5660-5667. doi: 10.1021/bi200438m

Fowler MW, Deweese JE, Dilks HH, Mercer SL, Phipps SD, McCormack J, Akers WS. An integrated biomedical and pharmaceutical sciences teaching laboratory for first-year PharmD students. Am J Pharm Educ. 2011;75(5):3. doi: 10.5688/ajpe755105

Schmidt BH, Burgin AB, Deweese JE, Osheroff N, Berger JM. A novel and unified two-metal mechanism for DNA cleavage by type II and IA topoisomerases. Nature. 2010;465(7298):641-644. doi: 10.1038/nature08974

Deweese JE, Osheroff N. The use of divalent metal ions by type II topoisomerases. Metallomics. 2010;2(7):450-459. doi: 10.1039/c003759a

Deweese JE, Osheroff N. Coordinating the two protomer active sites of human topoisomerase IIα: Nicks as topoisomerase II poisons. Biochemistry. 2009;48(7):1439-1441. doi: 10.1021/bi8021679

Deweese JE, Osheroff N. The DNA cleavage reaction of topoisomerase II: Wolf in sheep's clothing. Nucleic Acids Res. 2009;37(3):738-748. doi: 10.1093/nar/gkn937

Deweese JE, Guengerich FP, Burgin AB, Osheroff N. Metal ion interactions in the DNA cleavage/ligation active site of human topoisomerase IIα. Biochemistry. 2009;48(38):8940-8947. doi: 10.1021/bi900875c

Deweese JE, Burch AM, Burgin AB, Osheroff N. Use of divalent metal ions in the DNA cleavage reaction of human type II topoisomerases. Biochemistry. 2009;48(9):1862-1869. doi: 10.1021/bi8023256

Deweese JE, Osheroff MA, Osheroff N. DNA topology and topoisomerases: Teaching a "knotty" subject. Biochem Mol Biol Educ. 2008;37(1):2-10. doi: 10.1002/bmb.20244

Deweese JE, Burgin AB, Osheroff N. Human topoisomerase IIα uses a two-metal-ion mechanism for DNA cleavage. Nucleic Acids Res. 2008;36(15):4883-4893. doi: 10.1093/nar/gkn466

Deweese JE, Burgin AB, Osheroff N. Using 3'-bridging phosphorothiolates to isolate the forward DNA cleavage reaction of human topoisomerase IIα. Biochemistry. 2008;47(13):4129-4140. doi: 10.1021/bi702194x